Summary
Type: SPE
Application #: 300590
Catalog#: 730003 - SPE Cartridges, Chromabond, C18, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Step 1
Column conditioning:
3 ml methanol; dry column, then aspirate 3 ml 400 mM phosphate buffer (pH 10.5)
through the column
Sample application:
slowly force or aspitate the sample through the column (1)
Column washing:
force or aspirate 3 ml 400 mM phosphate buffer (pH 10.5), then 3 ml
methanol/dist. water (70:30, v/v) through the column
Elution:
3 ml methanol/dist. water (90:10, v/v); evaporate in a stream of nitrogen and
redissolve in 1 ml isopropanol/hexane (1:99, v/v)
Step 2:
Column conditioning:
force or aspirate 3 ml isopropanol/hexane ( 1:99, v/v) through the column
Sample application:
slowly force or aspirate the eluate from step 1 through the column
Column washing:
force or aspirate 10 ml isopropanol/hexane ( 3:97, v/v) through the column
Elution:
5 ml isopropanol/hexane (25:75, v/v); evaporate in a stream of nitrogen and
redissolve in 100 μl methanol
For further analyses we recommend HPLC.
Authors: LC Department
Source: Macherey-Nagel